This kit uses liquid nitrogen to fully grind plant tissues while using special reagents to precipitate the proteins released from plant cells, and uses a broad-spectrum protease inhibitor to inhibit protease activity to maximise the integrity of the extracted proteins, and the final protein extract is in the form of a dry powder, which is conducive to the long-term and stable preservation of proteins. The extracted proteins, including membrane proteins, nuclear proteins, cytoplasmic proteins and rare proteins contained in plant tissues including whole proteins. The protein fractions obtained from the isolation can be applied to SDS-PAGE, Western blot and so on.
The extracted proteins are whole plant cell proteins including rare proteins;
50 x 100 mg plant tissue protein extraction can be performed;
After appropriate treatment, it can be used for 2D electrophoresis, isoelectric point focusing and other experiments;
Good lysis effect on plant cells and removal of non-protein components of plant cells;
Does not require ultra-speed centrifugation and can process several samples at the same time.
Solution A 50mL
Solution B 100mL
Solution C 120μL
Solution D 1mL
Solution A/B 2~8℃ Solution C/D -20℃
For research use only, not for clinical use.