This kit is suitable for extracting soluble proteins from various plant seed samples with high starch content. Such as rice, buckwheat, maize, wheat and so on. The extraction process is simple and convenient.
This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The protein samples extracted by this kit contain high concentration of salt components and cannot be used directly for 2D electrophoresis."
Centrifuge the reagents in spin-cap centrifuge tubes by centrifuging briefly before opening the cap to shake the liquid on the inner wall of the cap to the bottom of the tube to avoid spilling the liquid when opening the cap.
All reagents during the experiment shall be pre-cooled; all apparatus shall be pre-cooled in a -20°C refrigerator. Samples should be kept at a low temperature throughout the process.
Protein extract A 25mL/50mL
Protein extract B 100mL/200mL
Protein extract C 25mL/50mL
Protein extract D 75mL/150mL
Protein Extract E 25mL/50mL
Extract A is stored at 2 to 8°C; extract BCDE is stored at room temperature.
For research use only, not for clinical use.