Plant Nuclear Protein Extraction Kit (Proteomic experiments, MS, enzymatic method)

This kit is suitable for the extraction of nuclear proteins from various plant cells and various solid plant tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nucleoproteins are not only of high purity and maintain natural activity, but also have no cross-contamination.
The kit contains a unique formula that can effectively solubilise plant nuclear components. The protease inhibitor mixture contained in this kit prevents the degradation of protein by protease, which ensures the extraction of high purity protein. The protein extraction components of this kit do not contain descaler components that cannot be removed by dialysis, and do not contain SDS, Triton X-100, chaps and other components that may affect the mass spectrometry experiments, and the protein samples obtained at the end of the dialysis or desalination will not contain descaler, high salt concentration and other influences, which basically satisfy the downstream of any proteomics-related experimental research.
The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the drift of mass spectrometry peaks due to AEBSF, so the protein samples extracted by this product can be used for mass spectrometry detection and analysis, proteomics (proteomics) and other related research.
The proteins extracted in this kit are active proteins with natural protein conformation. The kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The proteins extracted in this kit can also be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, and enzyme activity assay.

Contains protein stabiliser, the extracted protein is stable.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities, including serine proteases, cysteine proteases, aspartate proteases, alanyl-aminopeptidases and others.

Plant nuclear protein extract A 100mL/200mL
Protein extract B1 22.5mL/22.5mL
Protein extract B2 2.5mL/2.5mL
Nuclear Extract Solution C 25mL/50mL
Protease inhibitor mixture 100μL/200μL