Plant Nuclear Protein Extraction Kit (No detergents)

This kit is suitable for extracting nuclear proteins from various plant cells and various solid tissues, such as leaves, roots, seeds and other plant tissues. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nucleoproteins are not only of high purity and maintain natural activity, but also have absolutely no cross-contamination.
All components of this kit are free of detergent components, and the composition of the final protein sample has no significant impact on downstream applications such as NI column purification, molecular sieve, ion exchange, affinity purification, etc. This kit contains a unique formulation that can effectively solubilise plant nuclear components. This kit contains a mixture of protease inhibitors that prevents the degradation of proteins by proteases, providing for the extraction of highly pure proteins.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. The proteins extracted in this kit can be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking assay, enzyme activity assay and so on.
This kit adopts non-enzymatic extraction method, the extraction process is simple and convenient, and the speed can be completed within 1 hour.
The protein samples extracted by this kit contain high concentration of salt components and cannot be directly used for 2D electrophoresis.

Reduce the time of protein extraction to 1 hour.
Contains protein stabiliser for stable extracted protein.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The protease inhibitor mixture contains six separate protease inhibitors AEBSF, Aprotinin, Leupeptin, Pepstatin A, Bestatin, E-64, each of which specifically inhibits one or more protease activities. The optimised composition of the mixture allows it to inhibit almost all essential protease activities, including serine proteases, cysteine proteases, aspartate proteases, alanyl-aminopeptidases and others.

Plant nuclear protein extract A 50mL/100mL
Phycobiliprotein Extract B 50mL/100mL
Plant nucleoprotein extract C 15mL/30mL
Protease inhibitor mixture 250μL/500μL