Plant Membrane Protein Extraction Kit
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Plant Membrane Protein Extraction Kit

Cat: PE00009
Size: 50T|100T
Shelf Life: 1 year
Datasheet:

Application

This product is an efficient and high-yield membrane protein extraction kit that can extract total membrane proteins from various plants. It can be used for crude protein purification and membrane protein preparation. The extraction process is simple and convenient. This kit contains a mixture of protease inhibitors and phosphatase inhibitors, which prevents the degradation of proteins by proteases and ensures the extraction of high-quality proteins.
The protein extracted from this kit can be used in downstream protein research experiments such as WB, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel block test, enzyme activity determination, etc.
The protein extracted by this kit is an active protein with a natural protein conformation.
This reagent kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography. The protein samples extracted by this kit contain high concentrations of salt components and cannot be directly used for 2D electrophoresis

Description

Containing protein stabilizer, the extracted protein is stable.
When detecting protein concentration by UV, the background interference is low.
Protease inhibitors inhibit protein degradation, and the formulation of protease inhibitors is optimized.
The protease inhibitor mixture contains six independent protease inhibitors AEBSF, Aprotinin, Leupitin, Pepstatin A, Bestatin, E-64, each of which can specifically inhibit one or several protease activities. The optimized composition of this mixture enables it to inhibit almost all important protease activities, including serine protease, cysteine protease, aspartate protease, alanyl aminopeptidase, etc.

Kit Composition

Plant plasma membrane protein extract A 25mL/50mL
Phytoplasmic membrane protein extract B 250μ/500μL
Membrane protein lysate C 10mL/20mL
Protease inhibitor mixture 100μL/200μL"Protease Inhibitor Mixture 100μL/200μL"

Protein extracts and lysates are stored at 2~8℃; protease inhibitors are stored at -20℃.

For research use only, not for clinical use.