PIF4 | Phytochrome interacting factor 4 (goat antibody)

Inquiry

Cat: PA01356

Size: 50 µg

Host: Goat

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana PIF4, UniProt:Q8W2F3, TAIR:AT2G43010

Host

Goat

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

48,3 | 60 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica napus, Brassica rapa, Camelina sativa, Eutrema salsugineum

Not reactive in

Solanum lycopersicum

Additional information

PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light). Extraction of PIF proteins is described in Shen et al. (2007).,Material used need to be up to 8 days old as detection in older rosette leaf may fail

Description

PIF4(photochromin interaction Factor 4) is a transcription factor involved in the photochromin B signaling pathway. Interacting with APRR1/TOC1 and PIF3, combined with EGL2 and RGA. Aliases :AtPIF4, basic helix-loop-helix protein 9,AtbHLH9, bHLH9, SRL2, Short under red light 2, EN102, transcription factor EN102, bHLH transcription factor bHLH009.

For research use only, not for clinical use.