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Phototropin-1 (PHOT1), Recombinant Protein
Cat: RP04733
Size: 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Phototropin-1 (PHOT1) , partial
Product Gene Name
PHOT1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
111,689 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Phototropin
NCBI/Uniprot Data
NCBI Accession #
NP_001030814.1
NCBI GI #
79314333
NCBI GenBank Nucleotide #
NM_001035737.2
NCBI GeneID
823721
NCBI Official Full Name
phototropin 1
NCBI Official Symbol
PHOT1
NCBI Official Synonym Symbols
F16L2.3; JK224; NONPHOTOTROPIC HYPOCOTYL 1; NPH1; PHOTOTROPIN; phototropin 1; ROOT PHOTOTROPISM 1; RPT1
NCBI Protein Information
phototropin 1
NCBI Summary
Blue-light photoreceptor. Contains a light activated serine-threonine kinase domain and LOV1 and LOV2 repeats. Mutants are defective in blue-light response. Mediates blue light-induced growth enhancements. PHOT1 and PHOT2 mediate blue light-dependent activation of the plasma membrane H+-ATPase in guard cell protoplasts. PHOT1 undergoes blue-light-dependent autophosphorylation. At least eight phosphorylation sites have been identified in PHOT1. Phosphorylation of serine851 in the activation loop of PHOT1 appears to be required for stomatal opening, chloroplast accumulation, leaf flattening, and phototropism, and phosphorylation of serine849 may also contribute to the regulation of these responses. Phosphorylation-dependent binding of 14-3-3 proteins to the Hinge1 region of PHOT1 appears to require serine350 and serine376.
UniProt Gene Name
PHOT1
UniProt Synonym Gene Names
JK224; NPH1; RPT1
UniProt Protein Name
Phototropin-1
UniProt Synonym Protein Names
Non-phototropic hypocotyl protein 1; Root phototropism protein 1
UniProt Primary Accession #
O48963
UniProt Related Accession #
O48963
UniProt Comments
Protein kinase that acts as a blue light photoreceptor in a signal-transduction pathway for photo-induced movements. Phosphorylates BLUS1, a kinase involved in stomatal opening. Required for blue light mediated mRNA destabilization. Mediates calcium spiking of extracellular origin in response to a low rate of blue light. Also mediates rapid membrane depolarization and growth inhibition in response to blue light. Necessary for root phototropism. Involved in hypocotyl phototropism under a low rate but not under a high rate of blue light. Contributes to the chloroplast accumulation but seems not to be required for chloroplast translocation. Regulates stomata opening and photomorphogenesis response of leaf tissue. Confers sensitivity to drought. Not involved in hypocotyl elongation inhibition, anthocyanin accumulation or cotyledon opening.
For research use only, not for clinical use.