Modifications to backbone
                                                
                                                
                                                    gentamycin-resistant marker was removed and kanamycin-resistant marker (NOS promoter:NPTII:NOS terminator) was inserted.                                                
                                             
                                                                                    
                                                
                                                    Selectable markers
                                                
                                                
                                                    Kanamycin                                                
                                             
                                                                                    
                                                
                                                    Bacterial Resistance(s)
                                                
                                                
                                                    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL                                                
                                             
                                                                                    
                                                                                    
                                                
                                                    Growth Strain(s)
                                                
                                                
                                                    ccdB Survival