Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2), Recombinant Protein
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Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2), Recombinant Protein

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Cat: RP03593
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2), partial
Product Gene Name
FAD2 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
QHTHPSLPHY DSSEWDWLRG ALATVDRDYG ILNKVFHNIT DTHVAHHLFS TMPHYNAMEA TKAIKPILGD YYQFDGTPWY VAMYREAKEC IYVEPDREGD KKGVYWYNNK L
Sequence Positions
273-383. partial
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
44,048 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Delta(12)-oleate desaturase

NCBI/Uniprot Data

NCBI Accession #
NP_001319529.1
NCBI GI #
1063710968
NCBI GenBank Nucleotide #
NM_001337975.1
NCBI GeneID
820387
NCBI Official Full Name
fatty acid desaturase 2
NCBI Official Symbol
FAD2
NCBI Official Synonym Symbols
AtFAD2; DELTA-12 DESATURASE; fatty acid desaturase 2
NCBI Protein Information
fatty acid desaturase 2
NCBI Summary
Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
UniProt Gene Name
FAD2
UniProt Protein Name
Delta(12)-fatty-acid desaturase
UniProt Synonym Protein Names
Omega-6 fatty acid desaturase, endoplasmic reticulum
UniProt Primary Accession #
P46313
UniProt Related Accession #
P46313
UniProt Comments
ER (microsomal) omega-6 fatty acid desaturase introduces the second double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes (PubMed:14593172, PubMed:7907506). Delta(12)-desaturase with regioselectivity determined by the double bond (delta9 position) and carboxyl group of the substrate. Can use both 16:1 and 18:1 fatty acids as substrates (PubMed:8685264). It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine (PC) and, possibly, other phospholipids (PubMed:14593172, PubMed:7907506, PubMed:1730697). Very low constitutive hydroxylation activity (PubMed:11864983). Required for desaturation of fatty acids present in extraplastidial membranes, including mitochondria (PubMed:11104757, PubMed:17507388). Required for salt tolerance during seed germination and early seedling growth (PubMed:22279586).

For research use only, not for clinical use.