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Nitric oxide synthase 1 (NOS1), Recombinant Protein
Cat: RP02255
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Nitric oxide synthase 1 (NOS1) , partial
Product Gene Name
NOS1 recombinant protein
Product Synonym Gene Name
NOS1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
61,899 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Nitric oxide synthase
NCBI/Uniprot Data
NCBI Accession #
NP_190329.2
NCBI GI #
42565686
NCBI GenBank Nucleotide #
NM_114613.3
NCBI GeneID
823899
NCBI Official Full Name
P-loop containing nucleoside triphosphate hydrolases superfamily protein
NCBI Official Symbol
NOA1
NCBI Official Synonym Symbols
ATNOA1; ATNOS1; NITRIC OXIDE SYNTHASE 1; NO ASSOCIATED 1; NOS1; RESISTANT TO INHIBITION WITH FOSMIDOMYCIN 1; RESISTANT TO INHIBITION WITH FOSMIDOMYCIN 1; RIF1
NCBI Protein Information
P-loop containing nucleoside triphosphate hydrolases superfamily protein
NCBI Summary
Encodes a protein with similarity to the bacterial YqeH GTPase required for proper ribosome assembly. In Arabidopsis, mutant analyses show that this protein regulates growth and hormonal signaling in plants. It also attenuates oxidative stress and reactive oxygen species (ROS). It also seems to be involved in regulating leaf senescence and cell death. This gene product is also involved in nitric oxide biosynthesis in response to ABA but not exogenous H2O2. This protein also appears to be required for proper plastid biogenesis. Levels of several plastid-localized proteins, including RBCL, ClpP1, and the MEP biosynthesis enzymes DXS and DXR are altered in rif1-1 mutants. This protein was originally characterized as a mitrochondrial-localized nitric oxide synthase, but, the synthase activity was later disproven. In addition, new studies with GFP fusion proteins and chloroplast import assays suggest that this protein is found in chloroplasts.
UniProt Gene Name
NOA1
UniProt Synonym Gene Names
NOS1; RIF1; AtNOA1; AtNOS1
UniProt Protein Name
NO-associated protein 1, chloroplastic/mitochondrial
UniProt Synonym Protein Names
Dubious mitochondrial nitric oxide synthase 1 (EC:1.14.13.39); AtNOS1; GTPase NOA1; Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
UniProt Primary Accession #
Q66GP9
UniProt Secondary Accession #
Q0WWY8; Q157M8; Q9STX8; F4JBJ3
UniProt Related Accession #
Q66GP9
UniProt Comments
Exhibits cGTPase activity; binds and hydrolyzes specifically GTP (PubMed:18801746). May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl2and the presence of either KCl or (NH4)2SO4. Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.
For research use only, not for clinical use.