NEDD8-activating enzyme E1 catalytic subunit (ECR1), Recombinant Protein
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NEDD8-activating enzyme E1 catalytic subunit (ECR1), Recombinant Protein

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Cat: RP02595
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 catalytic subunit (ECR1)
Product Gene Name
ECR1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MADLDVPPQV PQSKTRDLDK LLLRHGNLVD PGFVPGPGLR DDIRDYVRIL VIGAGGLGCE LLKDLALSGF RNLEVIDMDR IEVTNLNRQF LFRIEDVGKP KAEVAAKRVM ERVSGVEIVP HFSRIEDKEI EFYNDFNIIA LGLDSIEARK YINGVACGFL EYNEDDTPKR ETIKPMVDGG TEGFKGHARV ILPGVTPCFE CTIYLFPPQV KFPLCTLAET PRNAAHCIEY AHLIQWETVH RGKTFDPDEP EHMKWVYDEA IRRAELFGIP GVTYSLTQGV VKNIIPAIAS TNAIISAACA LETLKIVSAC SKTLVNYLTY NGGEGLYTEV TKFERDTECL VCGPGILIEL DTSVTLSKFI EMLEDHPKLL LSKASVKQGE NTLYMQAPPV LEEFHRPKLS KPLYDLMGRV QKDTIHVFGQ RALKNNEKES CTTKVRVVFK GADGVADMDT AIGA
Sequence Positions
1-454, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
50,541 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_568370.1
NCBI GI #
18419850
NCBI GenBank Nucleotide #
NM_121923.4
NCBI GeneID
832038
NCBI Official Full Name
E1 C-terminal related 1
NCBI Official Symbol
ECR1
NCBI Official Synonym Symbols
E1 C-terminal related 1; T24G5.80; T24G5_80
NCBI Protein Information
E1 C-terminal related 1
NCBI Summary
Encodes a subunit of a RUB-activating enzyme analogous to the E1 ubiquitin-activating enzyme. ECR1 functions as a heterodimer with AXR1 to activate RUB, a ubiquitin-related protein.
UniProt Gene Name
ECR1
UniProt Protein Name
NEDD8-activating enzyme E1 catalytic subunit
UniProt Synonym Protein Names
RUB-activating enzyme; Ubiquitin-activating enzyme E1-like protein
UniProt Primary Accession #
O65041
UniProt Secondary Accession #
Q0WWG4; Q94A29
UniProt Related Accession #
O65041
UniProt Comments
Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1 .

For research use only, not for clinical use.