Mitogen-activated protein kinase 4 (MPK4), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 4 (MPK4)

Product Gene Name

MPK4 recombinant protein

Product Synonym Gene Name

MPK4

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MSAESCFGSS GDQSSSKGVA THGGSYVQYN VYGNLFEVSR KYVPPLRPIG RGAYGIVCAA TNSETGEEVA IKKIGNAFDN IIDAKRTLRE IKLLKHMDHE NVIAVKDIIK PPQRENFNDV YIVYELMDTD LHQIIRSNQP LTDDHCRFFL YQLLRGLKYV HSANVLHRDL KPSNLLLNAN CDLKLGDFGL ARTKSETDFM TEYVVTRWYR APELLLNCSE YTAAIDIWSV GCILGETMTR EPLFPGKDYV HQLRLITELI GSPDDSSLGF LRSDNARRYV RQLPQYPRQN FAARFPNMSA GAVDLLEKML VFDPSRRITV DEALCHPYLA PLHDINEEPV CVRPFNFDFE QPTLTEENIK ELIYRETVKF NPQDSV

Sequence Positions

1-376, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

42,852 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Mitogen-activated protein kinase

NCBI Accession #

NP_192046.1

NCBI GI #

15234152

NCBI GenBank Nucleotide #

NM_116367.3

NCBI GeneID

828151

NCBI Official Full Name

MAP kinase 4

NCBI Official Symbol

MPK4

NCBI Official Synonym Symbols

ATMPK4; F2N1.1; F2N1_1; MAP kinase 4; MAPK4

NCBI Protein Information

MAP kinase 4

NCBI Summary

Encodes a nuclear and cytoplasmically localized MAP kinase involved in mediating responses to pathogens. Its substrates include MKS1 and probably MAP65-1.The MAP65-1 interaction is involved in mediating cortical microtuble organization. Required for male-specific meiotic cytokinesis.

UniProt Gene Name

MPK4

UniProt Synonym Gene Names

AtMPK4; MAP kinase 4

UniProt Protein Name

Mitogen-activated protein kinase 4

UniProt Primary Accession #

Q39024

UniProt Secondary Accession #

O04597; Q45V20; Q9M136; B2BDE5; B2BDE6; B2BDE8; B2BDG2

UniProt Related Accession #

Q39024

UniProt Comments

The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. Essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during male-specific meiosis. Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Involved in root hair development process. Negative regulator of systemic acquired resistance (SAR) and salicylic acid- (SA) mediated defense response. Required for jasmonic acid- (JA) mediated defense gene expression. May regulate activity of transcription factor controlling pathogenesis-related (PR) gene expression. Seems to act independently of the SAR regulatory protein NPR1 (Nonexpresser of PR1). Phosphorylates MKS1 and transcription factors WRKY25 and WRKY33. The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity (PubMed:11163186, PubMed:15225555, PubMed:15358537, PubMed:15990873, PubMed:18982020, PubMed:20215588, PubMed:20802223, PubMed:21098735, PubMed:21575092, PubMed:25770109). Phosphorylates MEKK2 upon treatment with flg22 (PubMed:22643122).