Meiotic recombination protein SPO11-2 (SPO11-2), Recombinant Protein
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Meiotic recombination protein SPO11-2 (SPO11-2), Recombinant Protein

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Cat: RP08159
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Meiotic recombination protein SPO11-2 (SPO11-2)
Product Gene Name
SPO11-2 recombinant protein
Product Synonym Gene Name
SPO11-2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEESSGLSSM KFFSDQHLSY ADILLPHEAR ARIEVSVLNL LRILNSPDPA ISDLSLINRK RSNSCINKGI LTDVSYIFLS TSFTKSSLTN AKTAKAFVRV WKVMEICFQI LLQEKRVTQR ELFYKLLCDS PDYFSSQIEV NRSVQDVVAL LRCSRYSLGI MASSRGLVAG RLFLQEPGKE AVDCSACGSS GFAITGDLNL LDNTIMRTDA RYIIIVEKHA IFHRLVEDRV FNHIPCVFIT AKGYPDIATR FFLHRMSTTF PDLPILVLVD WNPAGLAILC TFKFGSIGMG LEAYRYACNV KWIGLRGDDL NLIPEESLVP LKPKDSQIAK SLLSSKILQE NYIEELSLMV QTGKRAEIEA LYCHGYNYLG KYIATKIVQG KYI
Sequence Positions
1-383, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
43,126 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Meiotic recombination protein

NCBI/Uniprot Data

NCBI Accession #
NP_176582.2
NCBI GI #
30696943
NCBI GenBank Nucleotide #
NM_105072.3
NCBI GeneID
842702
NCBI Official Full Name
sporulation 11-2
NCBI Official Symbol
SPO11-2
NCBI Official Synonym Symbols
F22C12.24; F22C12_24; sporulation 11-2
NCBI Protein Information
sporulation 11-2
NCBI Summary
Encodes AtSPO11-2, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. Plants homozygous for atspo11-2 exhibit a severe sterility phenotype. Both male and female meiosis are severely disrupted in the atspo11-2 mutant, and this is associated with severe defects in synapsis during the first meiotic division and reduced meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. Required for double-strand break induction.
UniProt Gene Name
SPO11-2
UniProt Synonym Gene Names
AtSPO11-2
UniProt Protein Name
Meiotic recombination protein SPO11-2
UniProt Primary Accession #
Q9M4A1
UniProt Secondary Accession #
Q9SH50
UniProt Related Accession #
Q9M4A1
UniProt Comments
Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:17018031, PubMed:17965269, PubMed:26917763).

For research use only, not for clinical use.