Meiotic recombination protein SPO11-1 (SPO11-1), Recombinant Protein
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Meiotic recombination protein SPO11-1 (SPO11-1), Recombinant Protein

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Cat: RP08499
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Meiotic recombination protein SPO11-1 (SPO11-1)
Product Gene Name
SPO11-1 recombinant protein
Product Synonym Gene Name
SPO11-1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEGKFAISES TNLLQRIKDF TQSVVVDLAE GRSPKISINQ FRNYCMNPEA DCLCSSDKPK GQEIFTLKKE PQTYRIDMLL RVLLIVQQLL QENRHASKRD IYYMHPSAFK AQSIVDRAIG DICILFQCSR YNLNVVSVGN GLVMGWLKFR EAGRKFDCLN SLNTAYPVPV LVEEVEDIVS LAEYILVVEK ETVFQRLAND MFCKTNRCIV ITGRGYPDVS TRRFLRLLME KLHLPVHCLV DCDPYGFEIL ATYRFGSMQM AYDIESLRAP DMKWLGAFPS DSEVYSVPKQ CLLPLTEEDK KRTEAMLLRC YLKREMPQWR LELETMLKRG VKFEIEALSV HSLSFLSEVY IPSKIRREVS SP
Sequence Positions
1-362, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
41,804 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Meiotic recombination protein

NCBI/Uniprot Data

NCBI Accession #
NP_187923.1
NCBI GI #
15231162
NCBI GenBank Nucleotide #
NM_112156.2
NCBI GeneID
820506
NCBI Official Full Name
Spo11/DNA topoisomerase VI, subunit A protein
NCBI Official Symbol
ATSPO11-1
NCBI Official Synonym Symbols
SPO11-1
NCBI Protein Information
Spo11/DNA topoisomerase VI, subunit A protein
NCBI Summary
Encodes AtSPO11-1, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. AtSPO11-1 accumulates in foci in early G2. At 1 h post-S phase, no foci are observed, but by 3 h a majority (80%) of meiocytes at this time point contain >50 foci. However, by 5 h, AtSPO11-1 foci are no longer detectable. This suggests that the protein undergoes a rapid cycle of accumulation and disappearance in meiocytes over a period of between 1 and 5 h post-S phase.
UniProt Gene Name
SPO11-1
UniProt Synonym Gene Names
AtSPO11-1
UniProt Protein Name
Meiotic recombination protein SPO11-1
UniProt Primary Accession #
Q9M4A2
UniProt Secondary Accession #
Q9C5S3; Q9LK56
UniProt Related Accession #
Q9M4A2
UniProt Comments
Component of a topoisomerase 6 complex specifically required for meiotic recombination (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763). Together with MTOPVIB, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917763). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles (PubMed:11157765, PubMed:17018031, PubMed:17965269, PubMed:26917763).

For research use only, not for clinical use.