Shipping
The extracted protein is provided in vials at a concentration of 5mg/ml and shipped on dry ice. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use.
Additional information
Protein quality can be examined by denaturing SDS-PAGE analysis, protein band clarity and resolution using Coomassie Blue staining. Protein integrity was examined by immunoblotting using a specific beta-actin antibody.
Application
Total protein is immediately available for Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gel and SDS - capillary electrophoresis, enzyme activity assays, gel shift assays, protein-protein interactions and tissue-specific expression.
Description
Maple total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.