Isoamylase 2, chloroplastic (ISA2), Recombinant Protein
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Isoamylase 2, chloroplastic (ISA2), Recombinant Protein

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Cat: RP02997
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Isoamylase 2, chloroplastic (ISA2) , partial
Product Gene Name
ISA2 recombinant protein
Product Synonym Gene Name
ISA2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
98,884 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Isoamylase

NCBI/Uniprot Data

NCBI Accession #
NP_171830.1
NCBI GI #
15218771
NCBI GenBank Nucleotide #
NM_100213.4
NCBI GeneID
839531
NCBI Official Full Name
debranching enzyme 1
NCBI Official Symbol
DBE1
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA ISOAMYLASE 2; AtBE2; ATISA2; BE2; BRANCHING ENZYME 2; debranching enzyme 1; F15K9.9; F15K9_9; ISA2
NCBI Protein Information
debranching enzyme 1
NCBI Summary
Encodes a protein with strong similarity to isoamylase (EC:3.2.1.68) however lacks critical residues known to be important for activity. Appears to co localize with ISA1 in the chloroplast isoamylase complex. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. It has been postulated that AtISA2 interacts with AtISA1 to form the Iso1 complex.
UniProt Gene Name
ISA2
UniProt Synonym Gene Names
DBE1; AtISA2
UniProt Protein Name
Isoamylase 2, chloroplastic
UniProt Synonym Protein Names
Protein DEBRANCHING ENZYME 1
UniProt Primary Accession #
Q8L735
UniProt Secondary Accession #
Q9ZVT2
UniProt Related Accession #
Q8L735
UniProt Comments
Involved in the trimming of pre-amylopectin chains. Accelerates the crystallization of nascent amylopectin molecules during starch synthesis. ISA1 and ISA2 work exclusively together as a multimeric holoenzyme. ISA1-ISA2 removes preferentially branches that are very close to other branches.

For research use only, not for clinical use.