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Indole-3-acetic acid-amido synthetase GH3.6 (GH3.6), Recombinant Protein
Cat: RP04657
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.6 (GH3.6) , partial
Product Gene Name
GH3.6 recombinant protein
Product Synonym Gene Name
GH3.6
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
68,897 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Indole-3-acetic acid-amido synthetase
NCBI/Uniprot Data
NCBI Accession #
NP_200262.1
NCBI GI #
15239653
NCBI GenBank Nucleotide #
NM_124831.3
NCBI GeneID
835539
NCBI Official Full Name
Auxin-responsive GH3 family protein
NCBI Official Symbol
DFL1
NCBI Official Synonym Symbols
DWARF IN LIGHT 1; F24B18.13; F24B18_13; GH3.6; Gretchen Hagen3.6
NCBI Protein Information
Auxin-responsive GH3 family protein
NCBI Summary
Encodes an IAA-amido synthase that conjugates Ala, Asp, Phe, and Trp to auxin. Lines overexpressing this gene accumulate IAA-ASP and are hypersensitive to several auxins. Identified as a dominant mutation that displays shorter hypocotyls in light grown plants when compared to wild type siblings. Protein is similar to auxin inducible gene from pea (GH3).
UniProt Gene Name
GH3.6
UniProt Synonym Gene Names
DFL1; AtGH3-6; DFL-1
UniProt Protein Name
Indole-3-acetic acid-amido synthetase GH3.6
UniProt Synonym Protein Names
Auxin-responsive GH3-like protein 6; AtGH3-6; Protein DWARF IN LIGHT 1; DFL-1
UniProt Primary Accession #
Q9LSQ4
UniProt Secondary Accession #
Q8VYV6
UniProt Related Accession #
Q9LSQ4
UniProt Comments
Catalyzes the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin. Strongly reactive with Glu, Gln, Trp, Asp, Ala, Leu, Phe, Gly, Tyr, Met, Ile and Val. Little or no product formation with His, Ser, Thr, Arg, Lys, or Cys. Also active on pyruvic and butyric acid analogs of IAA, PAA and the synthetic auxin naphthaleneacetic acid (NAA). The two chlorinated synthetic auxin herbicides 2,4-D and 3,6-dichloro-o-anisic acid (dicamba) cannot be used as substrates (PubMed:15659623). Involved in auxin signal transduction. Inhibits shoot and hypocotyl cell elongation, and lateral root cell differentiation in light (PubMed:11169197).
For research use only, not for clinical use.