Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), Recombinant Protein
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Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5), Recombinant Protein

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Cat: RP04199
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Indole-3-acetic acid-amido synthetase GH3.5 (GH3.5) , partial
Product Gene Name
GH3.5 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
69,283 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Indole-3-acetic acid-amido synthetase

NCBI/Uniprot Data

NCBI Accession #
NP_194456.1
NCBI GI #
15237038
NCBI GenBank Nucleotide #
NM_118860.5
NCBI GeneID
828834
NCBI Official Full Name
Auxin-responsive GH3 family protein
NCBI Official Symbol
WES1
NCBI Official Synonym Symbols
GH3.5; M4I22.70; M4I22_70
NCBI Protein Information
Auxin-responsive GH3 family protein
NCBI Summary
encodes an IAA-amido synthase that conjugates Asp and other amino acids to auxin in vitro. Lines carrying insertions in this gene are hypersensitive to auxin. It is involved in camalexin biosynthesis via conjugating indole-3-carboxylic acid (ICA) and cysteine (Cys).
UniProt Gene Name
GH3.5
UniProt Synonym Gene Names
AtGH3-5
UniProt Protein Name
Indole-3-acetic acid-amido synthetase GH3.5
UniProt Synonym Protein Names
Auxin-responsive GH3-like protein 5; AtGH3-5
UniProt Primary Accession #
O81829
UniProt Related Accession #
O81829
UniProt Comments
Catalyzes the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin. Strongly reactive with Glu, Gln, Trp, Asp, Ala, Leu, Phe, Gly, Tyr, Met, Ile and Val. Little or no product formation with His, Ser, Thr, Arg, Lys, or Cys. Also active on pyruvic and butyric acid analogs of IAA, PAA and the synthetic auxin naphthaleneacetic acid (NAA). The two chlorinated synthetic auxin herbicides 2,4-D and 3,6-dichloro-o-anisic acid (dicamba) cannot be used as substrates.

For research use only, not for clinical use.