IBA | Indole-3-butyric acid (rabbit antibody)

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Cat: PA01597

Size: 2,6 mg (at 2,681 mg/ml)

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Indole-3-butyric acid (IBA)

Datasheet:

Product Info

Immunogen

BSA-conjugated Indole-3-butyric acid, Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4(SIGMA), Solubility target: Methanol, ethanol.

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG. Protein A purified .

Format

Liquid

Storage

Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing.

Application

ELISA (ELISA), Immunoaffinity chromatography (IAC)

Recommended dilution

1 : 50 000 (indirect ELISA), 1 : 12 800 (direct ELISA)

Reactivity

Confirmed reactivity

Indole-3-butyric acid (IBA)

Additional information

As antibodies to BSA carrier are not removed from this preparation please do not use BSA in your assay and use OVA-conjugated IBA for coating in ELISA,The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralisation of the extract. Antibody titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance. Plates can be coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG can be used as detecting antibody.

Description

Indole-3-butyric acid (IBA) is a plant hormone belonging to the auxin group. It is used to stimulate root formation in plant cuttings.

For research use only, not for clinical use.