Titer in ELISA is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumine-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY is used as a tracer.As antibodies to BSA carrier are not removed from this preparation please use BSA in your assay and use OVA-conjugated IBA for coating in ELISA.
Indole-3-butyric acid (IBA) is a plant hormone belonging to the auxin group. It is used to stimulate root formation in plant cuttings.
For research use only, not for clinical use.