HY5 | Protein long hypocotyl 5

Inquiry

Cat: PA01207

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HY5 protein sequence.

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1: 500 to 1 : 1000 (WB)

Expected | apparent MW

18,5 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica pekinensis

Not reactive in

Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus sp. , Solanum lycopersicum, Triticum aestivum, Zea mays

Additional information

This antibody detects recombinant HY5 in Nicotiana benthamiana.Extraction and loading buffer with 6-8 M urea buffer needs to be used when working with endogenous extract to allow detection with this antibody or TCA/acetone protein extraction or as described in Mechin et al. (2007).

Description

HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in response to light. Involved in the blue light specific pathway. Heterodimer. It is expressed in roots, hypocotyls, cotyledon, leaves, stems and flower organs. Confined to the nucleus. Alias :Protein LONG HYPOCOTYL 5, bZIP transcription factor 56,AtbZIP56.

For research use only, not for clinical use.