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Histone-lysine N-methyltransferase MEDEA (MEA), Recombinant Protein
Cat: RP00278
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase MEDEA (MEA) , partial
Product Gene Name
MEA recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
79,310 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Histone-lysine N-methyltransferase
NCBI/Uniprot Data
NCBI Accession #
NP_563658.1
NCBI GI #
18378985
NCBI GenBank Nucleotide #
NM_100139.4
NCBI GeneID
839422
NCBI Official Full Name
SET domain-containing protein
NCBI Official Symbol
MEA
NCBI Official Synonym Symbols
EMB173; EMBRYO DEFECTIVE 173; FERTILIZATION INDEPENDENT SEED 1; FIS1; MEDEA; SDG5; SET DOMAIN-CONTAINING PROTEIN 5; T14P4.11; T14P4_11
NCBI Protein Information
SET domain-containing protein
NCBI Summary
Encodes a putative transcription factor MEDEA (MEA) that negatively regulates seed development in the absence of fertilization. Mutations in this locus result in embryo lethality. MEA is a Polycomb group gene that is imprinted in the endosperm. The maternal allele is expressed and the paternal allele is silent. MEA is controlled by DEMETER (DME), a DNA glycosylase required to activate MEA expression, and METHYLTRANSFERASE I (MET1), which maintains CG methylation at the MEA locus. MEA is involved in the negative regulation of its own imprinted gene expression; the effect is not only allele-specific but also dynamically regulated during seed development. In the ovule, the MEA transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization
UniProt Gene Name
MEA
UniProt Synonym Gene Names
EMB173; FIS1; MEDEA; SDG5; SET5
UniProt Protein Name
Histone-lysine N-methyltransferase MEDEA
UniProt Synonym Protein Names
Maternal embryogenesis control protein; Protein EMBRYO DEFECTIVE 173; Protein FERTILIZATION-INDEPENDENT SEED 1; Protein SET DOMAIN GROUP 5
UniProt Primary Accession #
O65312
UniProt Related Accession #
O65312
UniProt Comments
Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.
For research use only, not for clinical use.