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Histone-lysine N-methyltransferase ASHH2 (ASHH2), Recombinant Protein
Cat: RP04743
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2 (ASHH2) , partial
Product Gene Name
ASHH2 recombinant protein
Product Synonym Gene Name
ASHH2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
198,851 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Histone-lysine N-methyltransferase
NCBI/Uniprot Data
NCBI Accession #
NP_177854.6
NCBI GI #
240254387
NCBI GenBank Nucleotide #
NM_106379.9
NCBI GeneID
844066
NCBI Official Full Name
histone-lysine N-methyltransferase
NCBI Official Symbol
EFS
NCBI Official Synonym Symbols
ASH1 HOMOLOG 2; ASHH2; CAROTENOID CHLOROPLAST REGULATORY1; CCR1; EARLY FLOWERING IN SHORT DAYS; LAZ2; LAZARUS 2; SDG8; SET DOMAIN GROUP 8; T14N5.15; T14N5_15
NCBI Protein Information
histone-lysine N-methyltransferase
NCBI Summary
Encodes a protein with histone lysine N-methyltransferase activity required specifically for the trimethylation of H3-K4 in FLC chromatin (and not in H3-K36 dimethylation). Acts as an inhibitor of flowering specifically involved in the autonomous promotion pathway. EFS also regulates the expression of genes involved in carotenoid biosynthesis.Modification of histone methylation at the CRTISO locus reduces transcript levels 90%. The increased shoot branching seen in some EFS mutants is likely due to the carotenoid biosynthesis defect having an effect on stringolactones.Required for ovule, embryo sac, anther and pollen development.
UniProt Gene Name
ASHH2
UniProt Synonym Gene Names
H3-K36-HMTase 8
UniProt Protein Name
Histone-lysine N-methyltransferase ASHH2
UniProt Synonym Protein Names
ASH1 homolog 2
UniProt Primary Accession #
Q2LAE1
UniProt Secondary Accession #
O80663; Q56WW4; A9QA57
UniProt Comments
Histone methyltransferase involved in di and tri-methylation of 'Lys-36' of histone H3 (H3K36me2 and H3K36me3). Binds to H3 already mono- or di-methylated on 'Lys-4'(H3K4me1 or H3K4me2), but not to H3K4me3. H3K4me and H3K36me represent specific tags for epigenetic transcriptional activation. Regulates positively FLC transcription to prevent early flowering transition. Required for flowering transition in response to vernalization and for the maintenance of FLC expression in late embryos, but dispensable for the initial reactivation in early embryos during reprogramming. Seems also to modulate several traits including floral organ size, root size and dormancy. Promotes apical dominance (PubMed:16299497, PubMed:10518493, PubMed:16258034, PubMed:18070919, PubMed:19915673, PubMed:20711170). Directly involved in the tri-methylation of 'Lys-36' of histone H3 (H3K36me3) at LAZ5 chromatin to maintain a transcriptionally active state of LAZ5, a TIR-NB-LRR protein involved in innate immunity (PubMed:20949080). Required for brassinosteroid (BR)-induced gene expression and histone H3 trimethylation on 'Lys-36' (H3K36me3) in BR-regulated genes (PubMed:24838002).
For research use only, not for clinical use.