Histone H3.3, Recombinant Protein

Full Product Name

Recombinant Oryza coarctata Histone H3.3

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

ARTKQTARKS TGGKAPRKQL ATKAARKSAP TTGGVKKPHR YRPGTVALRE IRKYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSHAVL ALQEAAEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA

Sequence Positions

2-136, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

15,406 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Histone

NCBI Accession #

Q71U98.3

NCBI GI #

73919918

NCBI Official Full Name

Histone H3.3

UniProt Protein Name

Histone H3.3

UniProt Primary Accession #

Q71U98

UniProt Comments

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.