Histone H3.3 (OsI_011536), Recombinant Protein

Full Product Name

Recombinant Oryza sativa subsp. indica Histone H3.3 (OsI_011536)

Product Gene Name

OsI_011536 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

ARTKQTARKS TGGKAPRKQL ATKAARKSAP TTGGVKKPHR YRPGTVALRE IRKYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSHAVL ALQEAAEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA

Sequence Positions

2-136, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

15,406 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

A2XHJ3.1

NCBI GI #

158512877

NCBI Official Full Name

Histone H3.3

UniProt Gene Name

OsI_011536

UniProt Protein Name

Histone H3.3

UniProt Synonym Protein Names

H3.2

UniProt Primary Accession #

A2XHJ3

UniProt Secondary Accession #

Q71UF0; Q7F8L1; Q8SA80

UniProt Comments

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.