Histidine kinase 4 (AHK4), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Histidine kinase 4 (AHK4), partial

Product Gene Name

AHK4 recombinant protein

Product Synonym Gene Name

AHK4

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

117,955 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Histidine kinase

NCBI Accession #

NP_001324428.1

NCBI GI #

1063699527

NCBI GenBank Nucleotide #

NM_001335080.1

NCBI GeneID

814714

NCBI Official Full Name

CHASE domain containing histidine kinase protein

NCBI Official Symbol

WOL

NCBI Official Synonym Symbols

AHK4; ARABIDOPSIS HISTIDINE KINASE 4; ATCRE1; CRE1; CYTOKININ RESPONSE 1; T23K3.2; T23K3_2; WOL1; WOODEN LEG; WOODEN LEG 1

NCBI Protein Information

CHASE domain containing histidine kinase protein

NCBI Summary

Histidine kinase: cytokinin-binding receptor that transduces cytokinin signals across the plasma membrane

UniProt Gene Name

AHK4

UniProt Synonym Gene Names

CRE1; RAW1; WOL; AtHK4; AtCRE1; Cytokinin receptor CRE1

UniProt Protein Name

Histidine kinase 4

UniProt Synonym Protein Names

Arabidopsis histidine kinase 4; AtHK4; Cytokinin receptor CYTOKININ RESPONSE 1; AtCRE1; Cytokinin receptor CRE1; Phosphoprotein phosphatase AHK4 (EC:3.1.3.16); Protein AUTHENTIC HIS-KINASE 4; Protein ROOT AS IN WOL 1; Protein WOODEN LEG

UniProt Primary Accession #

Q9C5U0

UniProt Secondary Accession #

Q9C5T8; Q9C5T9; Q9FDZ3; Q9SIT0; A5YY60; A5YY75

UniProt Related Accession #

Q9C5U0

UniProt Comments

Cytokinins (CK) receptor related to bacterial two-component regulators. Binds also the synthetic urea-type cytokinin thiadiazuron, a potent defoliant and herbicide. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, removes phosphate from HPt proteins, decreasing the system phosphoload. Involved in meristems establishment in seedlings. Acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. Required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. Redundant positive regulator of cytokinin signaling that regulates many developmental processes including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), isopentenyladenine (iP), and isopentenyladenosine (iPR), the meta hydroxylated derivative of benzyladenine m-topolin, buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-butynyladenine (RM6), and to a lower extent, with cis-zeatin (cZ), zeatin riboside and dihydrozeatin (DZ). Together with AHK3, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Required for the formation of auxin-transporting vascular tissues in the hypocotyl, and primary and lateral roots, but not in adventitious roots, thus leading to auxin basipetal transport that regulates root development and branching. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Prevents the uptake of sulfate by mediating cytokinin-dependent down-regulation of high-affinity sulfate transporters (e.g. SULTR1;1 and SULTR1;2) expression in roots. Together with AHK2, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required to trigger the phytotoxic effect of the snapdragon (Antirrhinum majus) flowers volatile organic compound (VOC) methyl benzoate (MB). Plays a role in the cytokinin-mediated repression of the iron uptake pathway.