Histidine kinase 3 (AHK3), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Histidine kinase 3 (AHK3) , partial

Product Gene Name

AHK3 recombinant protein

Product Synonym Gene Name

AHK3

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

116,373 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Histidine kinase

NCBI Accession #

NP_564276.1

NCBI GI #

18396292

NCBI GenBank Nucleotide #

NM_102494.4

NCBI GeneID

839621

NCBI Official Full Name

histidine kinase 3

NCBI Official Symbol

HK3

NCBI Official Synonym Symbols

AHK3; F17L21.11; F17L21_11; histidine kinase 3

NCBI Protein Information

histidine kinase 3

NCBI Summary

Encodes a histidine kinases, a cytokinin receptor that controls cytokinin-mediated leaf longevity through a specific phosphorylation of the response regulator, ARR2.

UniProt Gene Name

AHK3

UniProt Synonym Gene Names

ORE12; AtHK3

UniProt Protein Name

Histidine kinase 3

UniProt Synonym Protein Names

Arabidopsis histidine kinase 3; AtHK3; Protein AUTHENTIC HIS-KINASE 3; Protein ORESARA 12

UniProt Primary Accession #

Q9C5U1

UniProt Secondary Accession #

Q9FZK3

UniProt Related Accession #

Q9C5U1

UniProt Comments

Cytokinins (CK) receptor related to bacterial two-component regulators. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. Involved in meristems establishment in seedlings. Redundant negative regulator of drought and salt stress responses and abscisic acid (ABA) signaling. Together with AHK2, plays a negative regulatory role in cold stress signaling via inhibition of ABA response, occurring independently of the cold acclimation pathway. Redundant positive regulator of cytokinin signaling that regulates many developmental processes including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), cis-zeatin (cZ), dihydrozeatin (DZ), buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-propynyladenine (RM3), 2-butynyladenine (RM6), and cytokinin ribosides and ribotides. Together with AHK4, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Promotes cytokinin-mediated leaf longevity through a specific phosphorylation of the response regulator ARR2. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Contributes to vascular bundle formation and secondary growth in a cytokinin-dependent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Plays a role in the cytokinin-mediated repression of the iron uptake pathway. Required by the cytokinin-dependent flower development regulation pathway.