HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Cat: PA00555

Size: 100 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata

Datasheet:

Product Info

Immunogen

Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein

Host

Mouse

Clonality

Monoclonal

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Liquid

Storage

Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Recommended dilution

1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)

Expected | apparent MW

78 kDa

Reactivity

Confirmed reactivity

Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata

Predicted reactivity

Higher plants

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml,Antibody in concentration 1 µg/ml is sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.

Description

HDEL (his-asp-glu-leu) is a C-terminal tripeptide found in yeast and plants that allows sorting of soluble proteins that reside in endoplasmic tubes.

For research use only, not for clinical use.