H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)
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H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)

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Cat: PA00869
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Human
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum.
Format
Liquid
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB)
Recommended dilution
0,5-1 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 20 000 (PA), 1 : 1000 (WB)
Expected | apparent MW
15 kDa

Reactivity

Confirmed reactivity
Human
Predicted reactivity
Arabidopsis thaliana, Caenorhabditis elegans, Mouse, Populus sp. , Solanum lycopersicum,  Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody is provided in PBS containing 0,05% azide and 0,05% ProClin 300

Description

Histone methylation and demethylation are dynamically regulated by histone methyltransferase and histone demethylase, respectively. Methylation of histone H3K27 is associated with inactive genomic regions.

For research use only, not for clinical use.