H3 | Histone H3 (antigen affinity purified)
  1. Home
  2. Products
  3. Plant Antibodies
  4. Plant General Antibodies
  5. Loading Controls
Products
Online Inquiry

H3 | Histone H3 (antigen affinity purified)

Inquiry
Cat: PA01001
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Oryza sativa
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ChIp-qPCR (ChIp-qPCR), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
2,5 µg/100 µg of chromatin (ChIp-qPCR), 1: 400 (IF), 1 : 5000 (WB)
Expected | apparent MW
15 | 17 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Oryza sativa
Predicted reactivity
Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Nannochloropsis gaditana, Triticum aestivum, Pinus pinaster, Pisum sativum, Zea mays, Vitis vinifera, Volvox sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii,Protocol for isolation of cytosolic and nuclear fractions can be found here. Specific protol for ChIP can be found here: Saleh et al. (2008).

Description

Histone 3 (H3) is located in the nucleus and incorporated into chromatin. It is present in nucleosomes along with H2A, H2B, and H4.

For research use only, not for clinical use.