Glyphosate

Antibody is provided in 0,01M PBS, pH 7,2. Composition of PBS: 8 mM Na2HPO4; 2 mM KH2PO4, 137 mM NaCl; 2,68 mM KCl.For coating OVA-glyphosate or KLH-glyphosate can be used.,This antibody is used to prepare anti-glyphosate immunoaffinity column which showed to be very specific and retained glyphosate but not AMPA or glufosinate. Recover of glyphosate is ca. 100 %. The antibody is applicable for any type of (waste) water sample. The detection limit is 2,5 μg/l, while the end concentration is 100 μg/l. The standard curve runs from 5 – 100 μg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assay buffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.

Glyphosate (N-(phosphorus methylethyl) glycine) is a broad-spectrum herbicide used to kill broadleaf weeds and grasses known to compete with cash crops around the world. Due to its physicochemical properties, glyphosate easily enters the aqueous environment and is quickly converted to its main metabolite, AMPA(aminomethylphosphonic acid).