GFP | Green fluorescent protein (VENUS)

Inquiry

Cat: PA00043

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: cell lysate overexoressing Venus protein fusion

Datasheet:

Product Info

Immunogen

Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

26 kDa

Reactivity

Confirmed reactivity

cell lysate overexoressing Venus protein fusion

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Description

The green fluorescent protein (Venus), from Aequorea victoria(jellyfish), can be mutated to emit at different wavelengths, such as blue for BFP (when tyr66 is replaced by His), cyan for CFP (when tyr66 is replaced by Trp) and yellow for YFP (when THR-203 is replaced by Tyr). It's an energy transfer receptor. Its role is to convert the blue chemiluminescence of the protein to green fluorescence through energy transfer.

For research use only, not for clinical use.