Ficin, from fig tree latex

Format

lyophilized powder

Storage

-20 °C

Concentration

≥1.0 units/mg protein

Additional information

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Solution

Protein, 80-100% biuret

Application

Ficin from fig tree latex has been used in the proteolysis of tendon collagen; in the digestion of Chlorophyta Ulva rigida; to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin.

Description

Ficin is classified as a thiol protease. It contains an active cysteine at the active site. The amino acid homology of the active site was similar to that of papain. Ficin will cut proteins on the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn and Val. The Km of the reported color developing substrate pglu-PH-Leu-p-nitroaniline is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethyl maleimide, mercury chloride, DFP(diisopropyl fluorophosphate), TLCK (na - to Toyl-lysine chloromethyl ketone), and TPCK (N-Toyl-l-phenylalanine chloromethyl ketone). Ficin can be used to generate high-yielding F (ab ')2 fragments from mouse IgG1.