EGFP | Enhanced Green Fluorescence Protein

Inquiry

Cat: PA00036

Size: 100 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: GFP, EGFP

Datasheet:

Product Info

Immunogen

Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified in PBS pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water, ultrapure water

Storage

Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened

Application

Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution

1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)

Expected | apparent MW

27 kDa (for GFP without fusion partner)

Reactivity

Confirmed reactivity

GFP, EGFP

Not reactive in

mCherry

Additional information

EGFP has Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000

Description

EGFP is an energy transfer receptor. Its role is to convert the blue chemiluminescence of the protein to green fluorescence through energy transfer. The Ca(2+) activated photoprotein aequorin fluoresces in the body when it receives energy. Contains chromophores composed of modified amino acid residues. The chromophore is formed by the autocatalytic main chain condensation between Xaa-N and Gly-(N+2) and the oxidation of Tyr-(N+1) to didehydrotyrosine. The chromophores only need molecular oxygen to mature.

For research use only, not for clinical use.