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(E,E)-alpha-farnesene synthase (TPS03), Recombinant Protein
Cat: RP03897
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana (E,E)-alpha-farnesene synthase , partial
Product Gene Name
TPS03 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
16,976 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
(E,E)-alpha-farnesene synthase
NCBI/Uniprot Data
NCBI Accession #
NP_001031651.1
NCBI GI #
79325133
NCBI GenBank Nucleotide #
NM_001036574.1
NCBI GeneID
827377
NCBI Official Full Name
terpene synthase 03
NCBI Official Symbol
TPS03
NCBI Official Synonym Symbols
ATTPS03; DL4395W; FCAALL.18; terpene synthase 03
NCBI Protein Information
terpene synthase 03
NCBI Summary
Encodes an (E,E)-alpha-farnesene synthase in the Col ecotype of Arabidopsis. This enzyme can also catalyze the formation of (E)-beta-ocimene as well as trace amounts of myrcene and other related compounds in vitro. The cytosolic localization of the protein may make it favor (E,E)-alpha-farnesene biosynthesis because the precursor of this product, FPP, is primarily cytosolic. Transcript levels for this gene increase in response to treatment with the jasmonic acid mimic coronalon or in response to the insect Plutella xylostella. TPS03 transcripts can also be detected in flowers. A similar protein from the C24 ecotype with one amino acid change (S267F) has a different substrate specificity.
UniProt Gene Name
TPS03
UniProt Synonym Gene Names
AtTPS03
UniProt Protein Name
Tricyclene synthase, chloroplastic
UniProt Synonym Protein Names
(E)-beta-ocimene synthase (EC:4.2.3.106); (E,E)-alpha-farnesene synthase (EC:4.2.3.46); Terpenoid synthase 3; AtTPS03
UniProt Primary Accession #
A4FVP2
UniProt Secondary Accession #
O23517; Q84NE5; Q8GWA8; Q8LE37; Q9FVI5
UniProt Related Accession #
A4FVP2
UniProt Comments
Predominantly involved in sesquiterpenes (C15) biosynthesis. Using FPP as substrate, the major product is (E,E)-alpha-farnesene with minor amounts of (Z,E)-alpha-farnesene and (E,E)-beta-farnesene. Using GPP as substrate, could also be able in vitro to synthesize monoterpene (C10) with (E)-beta-ocimene as the major product and with (Z)-beta-ocimene and myrcene as minor products.
For research use only, not for clinical use.