E3 ubiquitin-protein ligase RHF1A (RHF1A), Recombinant Protein
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E3 ubiquitin-protein ligase RHF1A (RHF1A), Recombinant Protein

Cat: RP04703
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RHF1A (RHF1A)
Product Gene Name
RHF1A recombinant protein
Product Synonym Gene Name
RHF1A
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MSNFTYTSAF NLSDNSPFNP AIGSSSSSSS ALVVASDDDN NTDDACSICL EPFTLQDPST VTSCKHEYHL QCIIEWSQRS KECPICWQLF VLRDPASQEL LAAVEKERLL KTRNISSSSP ISIHHSHDDF HSEEEESQFS SFDEQFLRHL TEAAHRRCLL RRRDGQISSS LVSSSDPTTI HPTDLVNLYR LSAISHVEHQ NSNPCPSPGS MTPSPVSGHS SIPADSNNGS RISPGPSPSR SSQSPKSPEA SSLPEAIKSK LAAASAKYKE SISKSKQGLK EKLLARNNSV KELSKGVQRE MNAGIAGVAR MIERMDFSSK RFGGSAHVST STATASGFNF SFKGKRVEAN SKSNNNGDKT EPQKLQGGET C
Sequence Positions
1-371, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
40,284 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
E3 ubiquitin-protein ligase

NCBI/Uniprot Data

NCBI Accession #
NP_193158.2
NCBI GI #
30682750
NCBI GenBank Nucleotide #
NM_117499.5
NCBI GeneID
827062
NCBI Official Full Name
RING-H2 group F1A
NCBI Official Symbol
RHF1A
NCBI Official Synonym Symbols
DL3150W; FCAALL.146; RING-H2 group F1A
NCBI Protein Information
RING-H2 group F1A
NCBI Summary
encodes a RING-type E3 ubiquitin ligase implicated in gametogenesis. RHF1a can interact with the cell cycle inhibitor ICK4/KRP6 in vitro. It apppears to target ICK4KRP6 for degradation following meiosis in order to allow the mitoses associated with megagametogenesis and microgametogenesis to occur. RHF1a is expressed in the carpels throughout floral development. It is expressed in various tissues of the anthers during the early stages of anther development but not in stage 12 flowers and beyond.
UniProt Gene Name
RHF1A
UniProt Protein Name
E3 ubiquitin-protein ligase RHF1A
UniProt Synonym Protein Names
RING-H2 finger F1a
UniProt Primary Accession #
Q4TU14
UniProt Secondary Accession #
O23280; Q8GXY4; Q9ZT43
UniProt Related Accession #
Q4TU14
UniProt Comments
E3 ubiquitin-protein ligase involved in the positive regulation of the gametogenesis progression. Mediates the proteasomal degradation of KRP6, a cyclin-dependent kinase inhibitor which accumulates during meiosis and blocks the progression of subsequent mitoses during gametophyte development. Functions in association with RHF2A (PubMed:18552199). Possesses E3 ubiquitin-protein ligase activity when associated with the E2 enzyme UBC8 in vitro (PubMed:15644464).

For research use only, not for clinical use.