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E3 ubiquitin-protein ligase ORTHRUS 2 (ORTH2), Recombinant Protein
Cat: RP05788
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 2 (ORTH2) , partial
Product Gene Name
ORTH2 recombinant protein
Product Synonym Gene Name
ORTH2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
71,266 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
E3 ubiquitin-protein ligase
NCBI/Uniprot Data
NCBI Accession #
NP_001319260.1
NCBI GI #
1063690130
NCBI GenBank Nucleotide #
NM_001333806.1
NCBI GeneID
842157
NCBI Official Full Name
Zinc finger (C3HC4-type RING finger) family protein
NCBI Official Symbol
VIM1
NCBI Official Synonym Symbols
F12K22.14; F12K22_14; ORTH2; ORTHRUS 2; VARIANT IN METHYLATION 1
NCBI Protein Information
Zinc finger (C3HC4-type RING finger) family protein
NCBI Summary
Encodes a 645-amino acid methylcytosine-binding protein with a PHD domain, two RING finger domains, and an SRA domain that is involved in centromere heterochromatinization. This protein functions as an E3 ubiquitin ligase in vitro. The protein has been shown to bind to methylated cytosines of CG, CNG and CNN motifs via its SRA domain but has a preference for the former. It plays a role in the establishment/maintenance of chromatin structure during cell division and is localized in the nucleus. Plants over-expressing VIM1/ORTH2 show an inhibition in root growth and a delay in flowering. Both over-expression of GFP:ORTH2 and loss of ORTH2/VIM1 lead to decreased levels of DNA methylation. GFP:ORTH2 over-expressers also have increased levels of FWA transcripts.
UniProt Gene Name
ORTH2
UniProt Synonym Gene Names
VIM1
UniProt Protein Name
E3 ubiquitin-protein ligase ORTHRUS 2
UniProt Synonym Protein Names
Protein VARIANT IN METHYLATION 1; RING-type E3 ubiquitin transferase ORTHRUS 2Curated
UniProt Primary Accession #
Q8VYZ0
UniProt Secondary Accession #
Q3ECM8; Q9FVS3
UniProt Related Accession #
Q8VYZ0
UniProt Comments
E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzyme UBC11. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Associates with methylated DNA, and can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate. Probably acts at the DNA methylation?histone interface to maintain centromeric heterochromatin.
For research use only, not for clinical use.