Products
Online Inquiry
E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1), Recombinant Protein
Cat: RP05693
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1) , partial
Product Gene Name
ORTH1 recombinant protein
Product Synonym Gene Name
ORTH1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
68,299 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
E3 ubiquitin-protein ligase
NCBI/Uniprot Data
NCBI Accession #
NP_198771.1
NCBI GI #
15241791
NCBI GenBank Nucleotide #
NM_123317.4
NCBI GeneID
833951
NCBI Official Full Name
Zinc finger (C3HC4-type RING finger) family protein
NCBI Official Symbol
VIM3
NCBI Official Synonym Symbols
MIJ24.3; MIJ24_3; ORTH1; ORTHRUS 1; VARIANT IN METHYLATION 3
NCBI Protein Information
Zinc finger (C3HC4-type RING finger) family protein
NCBI Summary
Encodes the VIM3/ORTH1 protein that is similar to VIM1. This protein has an N-terminal PHD domain and two RING domains surrounding an SRA domain. The protein has been shown to bind to methylated cytosines of CG, CNG and CNN motifs via its SRA domain but has a preference for the former. This protein functions as an E3 ubiquitin ligase in vitro with members of the UBC8 family E2s. Either of the two RING domains present in the protein can promote ubiquitylation in vitro, but, not the PHD domain. Over-expression of ORTH1/VIM3 leads to decreased levels of FWA methylation, increased levels of FWA transcripts, and delayed flowering. Cen180 repeats are also hypomethylated in plants overexpressing this protein.
UniProt Gene Name
ORTH1
UniProt Synonym Gene Names
VIM3
UniProt Protein Name
E3 ubiquitin-protein ligase ORTHRUS 1
UniProt Synonym Protein Names
Protein VARIANT IN METHYLATION 3; RING-type E3 ubiquitin transferase ORTHRUS 1Curated
UniProt Primary Accession #
Q9FKA7
UniProt Secondary Accession #
Q67XP0
UniProt Related Accession #
Q9FKA7
UniProt Comments
E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.
For research use only, not for clinical use.