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E3 ubiquitin-protein ligase CHIP (CHIP), Recombinant Protein
Cat: RP02391
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase CHIP (CHIP)
Product Gene Name
CHIP recombinant protein
Product Synonym Gene Name
CHIP
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MVTGVASAMA ERLKEDGNNC FKKERFGAAI DAYTEAIALS PNVPAYWTNR ALCHMKRKDW TKVEEDCRKA IQLVHNSVKA HYMLGLALLQ KKEFTNGVKE LQRALDLGRC SNPTGYMVEE IWEELSKAKY MEWELVSAMR SWELNSLKET CEAALNQQRA LDMSRTEESS DEAYTAHTER LKALERVFKK AAEEDKPTEV PDYLCCNITL EIFRDPVISP SGVTYERAAI LEHLKKVGKF DPITREKIDP ANLVPNLAIK EAVAAYLEKH VWAYKMGC
Sequence Positions
1-278, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
31,655 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Chitoporin
NCBI/Uniprot Data
NCBI Accession #
NP_566305.1
NCBI GI #
18397925
NCBI GenBank Nucleotide #
NM_111616.3
NCBI GeneID
819925
NCBI Official Full Name
carboxyl terminus of HSC70-interacting protein
NCBI Official Symbol
CHIP
NCBI Official Synonym Symbols
ATCHIP; carboxyl terminus of HSC70-interacting protein; CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN
NCBI Protein Information
carboxyl terminus of HSC70-interacting protein
NCBI Summary
Encodes AtCHIP, a new class of E3 ubiquitin ligases with three tetratricopeptide repeats and a U-box domain, structurally similar to the animal CHIP proteins. Plays an important role in plant cellular metabolism under temperature stress conditions. Functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment. Belongs to one of the 36 carboxylate clamp (CC)-tetratricopeptide repeat (TPR) proteins (Prasad 2010, Pubmed ID: 20856808) with potential to interact with Hsp90/Hsp70 as co-chaperones.
UniProt Gene Name
CHIP
UniProt Synonym Gene Names
AtCHIP
UniProt Protein Name
E3 ubiquitin-protein ligase CHIP
UniProt Synonym Protein Names
Carboxyl terminus of HSC70-interacting protein
UniProt Primary Accession #
Q9SRS9
UniProt Related Accession #
Q9SRS9
UniProt Comments
Has E3 ubiquitin-protein ligase activity and may target misfolded substrates towards proteasomal degradation. Regulates the activity of some serine/threonine-protein phosphatases by E3 ubiquitin-protein ligase activity. Required for responses to biotic and abiotic stresses such as auxin, abscisic acid (ABA), low and high temperature and darkness, probably through the activation of serine/threonine-protein phosphatase and the subsequent modification of the plasma membrane composition. Regulates the chloroplastic Clp proteolytic activity in response to stresses. Ubiquitylates FtsH1, a component of the chloroplast FtsH protease, and affects protein degradation in chloroplasts. Mediates plastid precursor degradation to prevent cytosolic precursor accumulation, together with the molecular chaperone HSC70-4. Mediates ubiquitination of transit peptides and thereby led to their degradation through the ubiquitin-proteasome system.
For research use only, not for clinical use.