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Dof zinc finger protein DOF5.5 (DOF5.5), Recombinant Protein
Cat: RP07153
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Dof zinc finger protein DOF5.5 (DOF5.5)
Product Gene Name
DOF5.5 recombinant protein
Product Synonym Gene Name
DOF5.5
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MLETKDPAIK LFGMKIPFPT VLEVADEEEE KNQNKTLTDQ SEKDKTLKKP TKILPCPRCN SMETKFCYYN NYNVNQPRHF CKACQRYWTS GGTMRSVPIG AGRRKNKNNS PTSHYHHVTI SETNGPVLSF SLGDDQKVSS NRFGNQKLVA RIENNDERSN NNTSNGLNCF PGVSWPYTWN PAFYPVYPYW SMPVLSSPVS SSPTSTLGKH SRDEDETVKQ KQRNGSVLVP KTLRIDDPNE AAKSSIWTTL GIKNEVMFNG FGSKKEVKLS NKEETETSLV LCANPAALSR SINFHEQM
Sequence Positions
1-298, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
26,696 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
NCBI/Uniprot Data
NCBI Accession #
NP_201049.3
NCBI GI #
145359559
NCBI GenBank Nucleotide #
NM_125637.4
NCBI GeneID
836364
NCBI Official Full Name
cycling DOF factor 1
NCBI Official Symbol
CDF1
NCBI Official Synonym Symbols
cycling DOF factor 1; K19B1.4; K19B1_4
NCBI Protein Information
cycling DOF factor 1
NCBI Summary
Dof-type zinc finger domain-containing protein, similar to H-protein promoter binding factor-2a GI:3386546 from (Arabidopsis thaliana). Represses expression of Constans (CO), a circadian regulator of flowering time. Interacts with LKP2 and FKF1. Expression oscillates under constant light conditions. Mainly expressed in the vasculature of cotyledons, leaves and hypocotyls, but also in stomata. Localized to the nucleus and acts as a repressor of CONSTANS through binding to the Dof binding sites in the CO promoter. Protein gets degraded by FKF1 in the afternoon.
UniProt Gene Name
CDF1
UniProt Synonym Gene Names
DOF5.5; AtDOF5.5
UniProt Protein Name
Cyclic dof factor 1
UniProt Synonym Protein Names
Dof zinc finger protein DOF5.5; AtDOF5.5
UniProt Primary Accession #
Q8W1E3
UniProt Secondary Accession #
Q4ACT9; Q9FJJ9
UniProt Related Accession #
Q8W1E3
UniProt Comments
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. A flanking TGT sequence contributes to the specificity of binding. Regulates a photoperiodic flowering response. Transcriptional repressor of 'CONSTANS' expression. The DNA-binding ability is not modulated by 'GIGANTEA' but the stability of CDF1 is controlled by the proteasome-dependent pathway. Ubiquitinated by the SCF(ADO3) E3 ubiquitin ligase complex. Binds to the FT promoter in the morning.
For research use only, not for clinical use.