DNA-directed RNA polymerase II subunit RPB7 (RPB19), Recombinant Protein
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DNA-directed RNA polymerase II subunit RPB7 (RPB19), Recombinant Protein

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Cat: RP04557
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB7 (RPB19)
Product Gene Name
RPB19 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MFFHIVLERN MQLHPRFFGR NLKENLVSKL MKDVEGTCSG RHGFVVAITG IDTIGKGLIR DGTGFVTFPV KYQCVVFRPF KGEILEAVVT LVNKMGFFAE AGPVQIFVSK HLIPDDMEFQ AGDMPNYTTS DGSVKIQKEC EVRLKIIGTR VDATAIFCVG TIKDDFLGVI NDPAAA
Sequence Positions
1-176, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
19,462 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_200726.1
NCBI GI #
15237831
NCBI GenBank Nucleotide #
NM_125308.4
NCBI GeneID
836036
NCBI Official Full Name
DNA-directed RNA polymerase II
NCBI Official Symbol
NRPB7
NCBI Official Synonym Symbols
MNC17.16; MNC17_16
NCBI Protein Information
DNA-directed RNA polymerase II
NCBI Summary
Non-catalytic subunit specific to DNA-directed RNA polymerase II; the ortholog of budding yeast RPB7
UniProt Gene Name
NRPB7
UniProt Synonym Gene Names
RPB19; RNA polymerase II subunit B7
UniProt Protein Name
DNA-directed RNA polymerase II subunit 7
UniProt Primary Accession #
P38421
UniProt Secondary Accession #
Q541V8
UniProt Related Accession #
P38421
UniProt Comments
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB7 is part of a subcomplex with NRPB4 that binds to a pocket formed by NRPB1, NRPB2 and NRPB6 at the base of the clamp element. The NRBP4-NRPB7 subcomplex seems to lock the clamp via NRPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The NRPB4-NRPB7 subcomplex binds single-stranded DNA and RNA .

For research use only, not for clinical use.