DNA-directed RNA polymerase II subunit RPB3-A (RPB36A), Recombinant Protein
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DNA-directed RNA polymerase II subunit RPB3-A (RPB36A), Recombinant Protein

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Cat: RP07182
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB3-A (RPB36A)
Product Gene Name
RPB36A recombinant protein
Product Synonym Gene Name
RPB36A
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MDGATYQRFP KIKIRELKDD YAKFELRETD VSMANALRRV MISEVPTVAI DLVEIEVNSS VLNDEFIAHR LGLIPLTSER AMSMRFSRDC DACDGDGQCE FCSVEFRLSS KCVTDQTLDV TSRDLYSADP TVTPVDFTID SSVSDSSEHK GIIIVKLRRG QELKLRAIAR KGIGKDHAKW SPAATVTFMY EPDIIINEDM MDTLSDEEKI DLIESSPTKV FGMDPVTRQV VVVDPEAYTY DEEVIKKAEA MGKPGLIEIS PKDDSFIFTV ESTGAVKASQ LVLNAIDLLK QKLDAVRLSD DTVEADDQFG ELGAHMRGG
Sequence Positions
1-319, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
35,461 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_179145.1
NCBI GI #
15226523
NCBI GenBank Nucleotide #
NM_127103.3
NCBI GeneID
816035
NCBI Official Full Name
DNA-directed RNA polymerase family protein
NCBI Official Symbol
NRPB3
NCBI Official Synonym Symbols
F26H6.5; F26H6_5; NRPD3; NRPE3A; RBP36A; RPB35.5A
NCBI Protein Information
DNA-directed RNA polymerase family protein
NCBI Summary
Non-catalytic subunit of nuclear DNA-dependent RNA polymerases II, IV and V; homologous to budding yeast RPB3 and the E. coli RNA polymerase alpha subunit. A closely related paralog, encoded by At2g15400, can substitute for At2g15430 in the context of Pol V.
UniProt Gene Name
NRPB3
UniProt Synonym Gene Names
NRPD3; NRPE3A; RPB36A; RNA polymerase II subunit 3-A; RNA polymerase II subunit B3-A
UniProt Protein Name
DNA-directed RNA polymerases II, IV and V subunit 3
UniProt Synonym Protein Names
DNA-directed RNA polymerase II 36 kDa polypeptide A; DNA-directed RNA polymerase II subunit RPB3-A; RNA polymerase II subunit 3-A; RNA polymerase II subunit B3-A
UniProt Primary Accession #
Q39211
UniProt Related Accession #
Q39211
UniProt Comments
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements.

For research use only, not for clinical use.