DNA-directed RNA polymerase II subunit RPB1 (RPB205), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB1 (RPB205) , partial

Product Gene Name

RPB205 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

204,626 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

NP_001329346.1

NCBI GI #

1063727065

NCBI GenBank Nucleotide #

NM_001342374.1

NCBI GeneID

829734

NCBI Official Full Name

RNA polymerase II large subunit

NCBI Official Symbol

NRPB1

NCBI Official Synonym Symbols

F4B14.70; F4B14_70; RNA polymerase II large subunit; RNA POLYMERASE II LARGE SUBUNIT; RNA_POL_II_LS; RNA_POL_II_LSRNA_POL_II_LS; RPB1

NCBI Protein Information

RNA polymerase II large subunit

NCBI Summary

Encodes the unique largest subunit of nuclear DNA-dependent RNA polymerase II; the ortholog of budding yeast RPB1 and a homolog of the E. coli RNA polymerase beta prime subunit.

UniProt Gene Name

NRPB1

UniProt Synonym Gene Names

RPB1; RPB205; RPII; DNA polymerase II subunit B1

UniProt Protein Name

DNA-directed RNA polymerase II subunit 1

UniProt Synonym Protein Names

DNA-directed RNA polymerase II subunit RPB1 (EC:2.7.7.6); DNA polymerase II subunit B1; DNA-directed RNA polymerase III largest subunit

UniProt Primary Accession #

P18616

UniProt Secondary Accession #

P31635; Q56Z04; Q9SZS8

UniProt Related Accession #

P18616

UniProt Comments

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from NRPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (NRPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.