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DNA-directed RNA polymerase D subunit 2a (NRPD2a), Recombinant Protein
Cat: RP06897
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana DNA-directed RNA polymerase D subunit 2a (NRPD2a) , partial
Product Gene Name
NRPD2a recombinant protein
Product Synonym Gene Name
NRPD2a
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
132,654 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
NCBI/Uniprot Data
NCBI Accession #
NP_001189957.1
NCBI GI #
334185578
NCBI GenBank Nucleotide #
NM_001203028.2
NCBI GeneID
821960
NCBI Official Full Name
nuclear RNA polymerase D2A
NCBI Official Symbol
NRPD2A
NCBI Official Synonym Symbols
DEFECTIVE IN MERISTEM SILENCING 2; DMS2; DRD2; NRPD2; NRPE2; nuclear RNA polymerase D2A; OCP1; OVEREXPRESSOR OF CATIONIC PEROXIDASE 1
NCBI Protein Information
nuclear RNA polymerase D2A
NCBI Summary
This gene encodes the second largest, catalytic subunit of the nuclear DNA-dependent RNA polymerase IV (aka RNA polymerase D). The NRPD2 protein is found at nuclear foci that overlap or are adjacent to chromocentromeres but are not fully coincident with chromocentromeres. The loss of NRPD2 leads to the loss of cytosine methylation at pericentromeric 5S genes and AtSN1 retroelements but has no discernible effect on centromere repeat methylation. This suggests that Pol IV primarily affects facultative heterochromatin rather than constitutive heterochromatin.
UniProt Gene Name
NRPD2
UniProt Synonym Gene Names
DMS2; DRD2; NRPD2a; NRPE2; RPD2a; AtNRPD2a; Nuclear RNA polymerase D 2a; POL IV 2a
UniProt Protein Name
DNA-directed RNA polymerases IV and V subunit 2
UniProt Synonym Protein Names
DNA-directed RNA polymerase D subunit 2a; AtNRPD2a; Nuclear RNA polymerase D 2a; Nuclear RNA polymerase E 2; Protein DEFECTIVE IN MERISTEM SILENCING 2; Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 2; RNA polymerase IV subunit 2a; POL IV 2a
UniProt Primary Accession #
Q9LK40
UniProt Secondary Accession #
Q67ZK6
UniProt Related Accession #
Q9LK40
UniProt Comments
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Also required for full erasure of methylation when the RNA trigger is withdrawn. Required for intercellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing. During interphase, mediates siRNA-independent heterochromatin association and methylation into chromocenters and condensation and cytosine methylation at pericentromeric major repeats. Required for complete maintenance of the 35S promoter homology-dependent TGS in transgenic plants and for the initial establishment of DNA methylation.
For research use only, not for clinical use.