DISCONTINUED HSP23,5 | Heat shock protein 23,5 (mitochondrial)
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DISCONTINUED HSP23,5 | Heat shock protein 23,5 (mitochondrial)

Cat: PA00229
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana recombinant HSP23.5
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HSP23.5, UniProt: Q9FGM9, TAIR: AT5G51440
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7,4
Format
Lyophilized
Reconstitution
For reconstitution add 25 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Application
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
23,5 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana recombinant HSP23.5
Predicted reactivity
Arabidopsis thaliana
Not reactive in
Chlamydomonas reinhardtii, Zea mays

Additional information

This antibody is recognizing recombinant HSP23.5 and reactivity on endogenous protein needs to be confirmed. So far HSP23.5 could not be detected in whole seedling extracts under conditions used to detect HSP23.6. High sensitivity reagents and enrichment are necessary due to low amounts of HSP23.5 in endogenous tissue.Antibody is not cross reacting with cytosolic HSPs.,Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C. Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

Description

HSP23.5 | Heat shock protein 23.5(mitochondria) belongs to the small heat shock protein family HSP20.

For research use only, not for clinical use.