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Cytochrome P450 90B1 (CYP90B1), Recombinant Protein
Cat: RP08220
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1) , partial
Product Gene Name
CYP90B1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
58,868 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Cytochrome
NCBI/Uniprot Data
NCBI Accession #
NP_190635.1
NCBI GI #
15229822
NCBI GenBank Nucleotide #
NM_114926.4
NCBI GeneID
824229
NCBI Official Full Name
Cytochrome P450 superfamily protein
NCBI Official Symbol
DWF4
NCBI Official Synonym Symbols
AtDWF4; CLM; CLOMAZONE-RESISTANT; CYP90B1; CYTOCHROME P450 90B1; DWARF 4; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PARTIALLY SUPPRESSING COI1 INSENSITIVITY TO JA 1; PSC1; SAV1; SHADE AVOIDANCE 1; SNP2; STEROID 22-ALPHA-HYDROXYLASE; SUPPRESSOR OF
NCBI Protein Information
Cytochrome P450 superfamily protein
NCBI Summary
Encodes a 22alpha hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate.
UniProt Gene Name
CYP90B1
UniProt Synonym Gene Names
DWF4; Dwarf4
UniProt Protein Name
Cytochrome P450 90B1
UniProt Synonym Protein Names
Protein DWARF 4; Dwarf4; Steroid 22-alpha-hydroxylase
UniProt Primary Accession #
O64989
UniProt Secondary Accession #
Q9SCQ9
UniProt Related Accession #
O64989
UniProt Comments
Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.
For research use only, not for clinical use.