Cytochrome P450 85A2 (CYP85A2), Recombinant Protein
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Cytochrome P450 85A2 (CYP85A2), Recombinant Protein

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Cat: RP07956
Size: 0.5 mg (E-Coli)/ 0.05 mg (Baculovirus)/ 0.5 mg (Yeast)/ 0.05 mg (Mammalian-Cell)/ 1 mg (E-Coli)/ 0.1 mg (Baculovirus)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Cytochrome P450 85A2 (CYP85A2), partial
Product Gene Name
CYP85A2 recombinant protein
Product Synonym Gene Name
CYP85A2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MGIMMMILGL LVIIVCLCTA LLRWNQMRYS KKGLPPGTMG WPIFGETTEF LKQGPDFMKN QRLRYGSFFK SHILGCPTIV SMDAELNRYI LMNESKGLVA GYPQSMLDIL GTCNIAAVHG PSHRLMRGSL LSLISPTMMK DHLLPKIDDF MRNYLCGWDD LETVDIQEKT KHMAFLSSLL QIAETLKKPE VEEYRTEFFK LVVGTLSVPI DIPGTNYRSG VQARNNIDRL LTELMQERKE SGETFTDMLG YLMKKEDNRY LLTDKEIRDQ VVTILYSGYE TVSTTSMMAL KYLHDHPKAL EELRREHLAI RERKRPDEPL TLDDIKSMKF TRAVIFETSR LATIVNGVLR KTTHDLELNG YLIPKGWRIY VYTREINYDT SLYEDPMIFN PWRWMEKSLE SKSYFLLFGG GVRLCPGKEL GISEVSSFLH YFVTKYRWEE NGEDKLMVFP RVSAPKGYHL KCSPY
Sequence Positions
1-465
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
53,815 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Cytochrome

NCBI/Uniprot Data

NCBI Accession #
NP_566852.1
NCBI GI #
18406372
NCBI GenBank Nucleotide #
NM_113917.4
NCBI GeneID
822709
NCBI Official Full Name
brassinosteroid-6-oxidase 2
NCBI Official Symbol
BR6OX2
NCBI Official Synonym Symbols
brassinosteroid-6-oxidase 2; CYP85A2
NCBI Protein Information
brassinosteroid-6-oxidase 2
NCBI Summary
Encodes a cytochrome p450 enzyme that catalyzes the last reaction in the production of brassinolide. It is capable of converting 6-deoxocastasterone into castasterone, a C-6 oxidation, as well as the further conversion of castasterone into brassinolide by a Baeyer-Villinger oxidation reaction at C-6, resulting in the formation of an unusual seven-membered lactone ring. The enzyme possesses high affinity for both C28- and C27-Brassinosteroids. The expression of the gene using a CYP85A2 promoter:LUC fusion construct was shown to be under circadian and light control.
UniProt Gene Name
CYP85A2
UniProt Synonym Gene Names
BR6OX2; BR6ox 2
UniProt Protein Name
Cytochrome P450 85A2
UniProt Synonym Protein Names
Brassinosteroid-6-oxidase 2; BR6ox 2; C6-oxidase 2
UniProt Primary Accession #
Q940V4
UniProt Secondary Accession #
Q8LFX3; Q9LH81
UniProt Related Accession #
Q940V4
UniProt Comments
Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. Converts 6-deoxocastasterone to castasterone, and castasterone to brassinolide. May also convert 6-deoxoteasterone to teasterone, 3-dehydro-6-deoxoteasterone to 3-dehydroteasterone, and 6-deoxotyphasterol to typhasterol.

For research use only, not for clinical use.