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Cysteine protease ATG4b (ATG4B), Recombinant Protein
Cat: RP05660
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Cysteine protease ATG4b (ATG4B)
Product Gene Name
ATG4B recombinant protein
Product Synonym Gene Name
ATG4B
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MKAICDRFVP SKCSSSSTSE KRDISSPTSL VSDSASSDNK SNLTLCSDVV ASSSPVSQLC REASTSGHNP VCTTHSSWTV ILKTASMASG AIRRFQDRVL GPSRTGISSS TSEIWLLGVC YKISEGESSE EADAGRVLAA FRQDFSSLIL MTYRRGFEPI GDTTYTSDVN WGCMLRSGQM LFAQALLFQR LGRSWRKKDS EPADEKYLEI LELFGDTEAS AFSIHNLILA GESYGLAAGS WVGPYAVCRS WESLARKNKE ETDDKHKSFS MAVHIVSGSE DGERGGAPIL CIEDVTKTCL EFSEGETEWP PILLLVPLVL GLDRVNPRYI PSLIATFTFP QSLGILGGKP GASTYIVGVQ EDKGFYLDPH DVQQVVTVKK ENQDVDTSSY HCNTLRYVPL ESLDPSLALG FYCQHKDDFD DFCIRATKLA GDSNGAPLFT VTQSHRRNDC GIAETSSSTE TSTEISGEEH EDDWQLL
Sequence Positions
1-477, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
29,876 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Cysteine protease
NCBI/Uniprot Data
NCBI Accession #
NP_001118859.1
NCBI GI #
186511209
NCBI GenBank Nucleotide #
NM_001125387.1
NCBI GeneID
825165
NCBI Official Full Name
Peptidase family C54 protein
NCBI Official Symbol
AT3G59950
NCBI Protein Information
Peptidase family C54 protein
UniProt Gene Name
ATG4B
UniProt Synonym Gene Names
APG4B; AtAPG4b; Protein autophagy 4b
UniProt Protein Name
Cysteine protease ATG4b
UniProt Synonym Protein Names
Autophagy-related protein 4 homolog b; AtAPG4b; Protein autophagy 4b
UniProt Primary Accession #
Q9M1Y0
UniProt Secondary Accession #
Q570T6
UniProt Related Accession #
Q9M1Y0
UniProt Comments
Cysteine protease required for autophagy, which is able to cleave the C-terminal part of ATG8 that may be subsequently converted to a smaller form, with a revealed C-terminal glycine. ATG8 is then activated by ATG7 and is subsequently attached to ATG3, another E2-like enzyme, after which the C-terminal glycine of ATG8 is conjugated to phosphatidylethanolamine by an amide bond. This conjugated form has the capacity for the binding to autophagosomes.
For research use only, not for clinical use.