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Cysteine protease ATG4a (ATG4A), Recombinant Protein
Cat: RP05090
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Cysteine protease ATG4a (ATG4A)
Product Gene Name
ATG4A recombinant protein
Product Synonym Gene Name
ATG4A
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MKALCDRFVP QQCSSSSKSD THDKSPLVSD SGPSDNKSKF TLWSNVFTSS SSVSQPYRES STSGHKQVCT TRNGWTAFVK RVSMASGAIR RFQERVLGPN RTGLPSTTSD VWLLGVCYKI SADENSGETD TGTVLAALQL DFSSKILMTY RKGFEPFRDT TYTSDVNWGC MIRSSQMLFA QALLFHRLGR AWTKKSELPE QEYLETLEPF GDSEPSAFSI HNLIIAGASY GLAAGSWVGP YAICRAWESL ACKKRKQTDS KNQTLPMAVH IVSGSEDGER GGAPILCIED ATKSCLEFSK GQSEWTPIIL LVPLVLGLDS VNPRYIPSLV ATFTFPQSVG ILGGKPGAST YIVGVQEDKG FYLDPHEVQQ VVTVNKETPD VDTSSYHCNV LRYVPLESLD PSLALGFYCR DKDDFDDFCL RALKLAEESN GAPLFTVTQT HTAINQSNYG FADDDSEDER EDDWQML
Sequence Positions
1-467, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
46,875 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Cysteine protease
NCBI/Uniprot Data
NCBI Accession #
NP_001318423.1
NCBI GI #
1063703263
NCBI GenBank Nucleotide #
NM_001337075.1
NCBI GeneID
819020
NCBI Official Full Name
Peptidase family C54 protein
NCBI Official Symbol
AT2G44140
NCBI Official Synonym Symbols
F6E13.27
NCBI Protein Information
Peptidase family C54 protein
UniProt Gene Name
ATG4A
UniProt Synonym Gene Names
APG4A; AtAPG4a; Protein autophagy 4a
UniProt Protein Name
Cysteine protease ATG4a
UniProt Synonym Protein Names
Autophagy-related protein 4 homolog a; AtAPG4a; Protein autophagy 4a
UniProt Primary Accession #
Q8S929
UniProt Secondary Accession #
O80583; Q3EBG5
UniProt Related Accession #
Q8S929
UniProt Comments
Cysteine protease required for autophagy, which is able to cleave the C-terminal part of ATG8 that may be subsequently converted to a smaller form, with a revealed C-terminal glycine. ATG8 is then activated by ATG7 and is subsequently attached to ATG3, another E2-like enzyme, after which the C-terminal glycine of ATG8 is conjugated to phosphatidylethanolamine by an amide bond. This conjugated form has the capacity for the binding to autophagosomes.
For research use only, not for clinical use.