Cyclic nucleotide-gated ion channel 2 (CNGC2) (DND1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Cyclic nucleotide-gated ion channel 2 (CNGC2)

Product Synonym Names

Recombinant Cyclic nucleotide-gated ion channel 2 (CNGC2); Cyclic nucleotide-gated ion channel 2; AtCNGC2; Cyclic nucleotide- and calmodulin-regulated ion channel 2 Protein DEFENSE NO DEATH 1

Product Gene Name

DND1 recombinant protein

Product Synonym Gene Name

CNGC2; DND1

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MPSHPNFIFR WIGLFSDKFR RQTTGIDENS NLQINGGDSS SSGSDETPVL SSVECYACTQ VGVPAFHSTS CDQAHAPEWR ASAGSSLVPI QEGSVPNPAR TRFRRLKGPF GEVLDPRSKR VQRWNRALLL ARGMALAVDP LFFYALSIGR TTGPACLYMD GAFAAVVTVL RTCLDAVHLW HVWLQFRLAY VSRESLVVGC GKLVWDPRAI ASHYARSLTG FWFDVIVILP VPQAVFWLVV PKLIREEKVK LIMTILLLIF LFQFLPKIYH CICLMRRMQK VTGYIFGTIW WGFALNLIAY FIASHVAGGC WYVLAIQRVA SCIRQQCMRT GNCNLSLACK EEVCYQFVSP TSTVGYPCLS GNLTSVVNKP MCLDSNGPFR YGIYRWALPV ISSNSLAVKI LYPIFWGLMT LSTFANDLEP TSNWLEVIFS IVMVLSGLLL FTLLIGNIQV FLHAVMAKKR KMQIRCRDME WWMKRRQLPS RLRQRVRRFE RQRWNALGGE DELELIHDLP PGLRRDIKRY LCFDLINKVP LFRGMDDLIL DNICDRAKPR VFSKDEKIIR EGDPVQRMIF IMRGRVKRIQ SLSKGVLATS TLEPGGYLGD ELLSWCLRRP FLDRLPPSSA TFVCLENIEA FSLGSEDLRY ITDHFRYKFA NERLKRTARY YSSNWRTWAA VNIQMAWRRR RKRTRGENIG GSMSPVSENS IEGNSERRLL QYAAMFMSIR PHDHLE

Sequence Positions

1-726

Chromosome Location

Chromosome: 5; NC_003076.8 (5003307..5006882, complement). Location: chromosome: 5

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

83,241 Da

Storage

Store at -20℃. For extended storage, store at -20 or -80℃.

Protein Family

Dead end protein

NCBI Accession #

NP_197045.1

NCBI GI #

15242291

NCBI GenBank Nucleotide #

NM_121545.4

NCBI GeneID

831393

NCBI Official Full Name

cyclic nucleotide-gated ion channel 2

NCBI Official Symbol

DND1

NCBI Official Synonym Symbols

ATCNGC2; CNGC2; CYCLIC NUCLEOTIDE GATED CHANNEL 2; CYCLIC NUCLEOTIDE-GATED CHANNEL 2; DEFENSE NO DEATH 1; DND1

NCBI Protein Information

cyclic nucleotide-gated ion channel 2

UniProt Gene Name

CNGC2

UniProt Synonym Gene Names

DND1; AtCNGC2

UniProt Protein Name

Cyclic nucleotide-gated ion channel 2

UniProt Synonym Protein Names

Cyclic nucleotide- and calmodulin-regulated ion channel 2; Protein DEFENSE NO DEATH 1

UniProt Entry Name

CNGC2_ARATH

UniProt Primary Accession #

O65718

UniProt Related Accession #

O65718

UniProt Comments

Function: Acts as cyclic nucleotide-gated ion channel. Permeable to potassium and calcium in a cyclic nucleotide-dependent fashion (cAMP or cGMP). Could also transport lithium, cesium and rubium and displays a strong selectivity against sodium. Seems to directly participate in pathogen-induced calcium influx. May function in homeostasis, re-establishing ionic balance after defense action and/or other stimuli. Could mediate the initiation of the developmentally regulated cell death programs.Subunit structure: Homotetramer or heterotetramer Potential. Binds calmodulin-1/4 with a higher affinity than calmodulin-2/3/5.Subcellular location: Cell membrane; Multi-pass membrane protein Potential. Tissue specificity: Expressed in the whole plant but only weakly in roots. Strongly expressed in the expanded cotyledons of 14-day-old seedlings and detected later in leaves after the transition to flowering. Also detected in flowers during organ senescence and in the dehiscence zone of siliques.Induction: Up-regulated by light. Transiently induced during leaf and culture senescence.Domain: The binding of calmodulin to the C-terminus might interfere with cyclic nucleotide binding and thus channel activation By similarity.Miscellaneous: Loss-of-function mutations cngc2-1 (dnd1-1) or cncg2-2 results in the loss of the hypersensitive response and leads to a broad spectrum disease resistance. These mutations lead to a specific and dramatic calcium hypersensitivity that results in severe reductions in plant size and seed yield.Sequence similarities: Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. Contains 1 cyclic nucleotide-binding domain.Contains 1 IQ domain.